Colorimetric immunoassays using gold nanoparticles (AuNP) form a special class of assays where AuNP act as a transducer to monitor binding events between an antigen and an antibody. Indeed, AuNP… Click to show full abstract
Colorimetric immunoassays using gold nanoparticles (AuNP) form a special class of assays where AuNP act as a transducer to monitor binding events between an antigen and an antibody. Indeed, AuNP display unique optical properties that can been exploited in various ways to develop biosensors. One of the most striking properties of colloidal AuNP (and more generally of noble metal nanomaterials) is their extremely high extinction coefficient in the visible range of the spectrum owing to the localized surface plasmon resonance (LSPR) phenomenon. This feature makes AuNP detectable down to very low concentrations by absorption spectroscopy or even by the naked eye. Herein we took advantage of the high detectability of AuNP to design a solid-phase, sandwich-type, colorimetric immunosensor aiming at the detection of staphylococcal enterotoxin A (SEA). A test zone comprised of a polyclonal anti-SEA antibody was created at the surface of amino-functionalized glass slides via high affinity binding to covalently immo...
               
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