LAUSR

Detection of specific oligonucleotide sequences is central to numerous applications and technologies amenable to point-of-care diagnostics or end users are needed. Here we report a technology making use of a biolumi-nescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conju-gate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarker, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.