Prompted by a knowledge of the photoprotective mechanism operating in photosystem supercomplexes and bacterial antenna complexes by pigment binding proteins, we have appealed to a box-like synthetic receptor (ExBox•4Cl) which… Click to show full abstract
Prompted by a knowledge of the photoprotective mechanism operating in photosystem supercomplexes and bacterial antenna complexes by pigment binding proteins, we have appealed to a box-like synthetic receptor (ExBox•4Cl) which binds a photosensitizer, 5,15-diphenylporphyrin (DPP) to provide photoprotection by regulating light energy. The hydrophilic ExBox4+ renders DPP soluble in water, and modulates the phototoxicity of DPP by trapping it in its cavity and releasing it when required. While trapping removes access to the DPP triplet state, a pH-dependent release of diprotonated DPP (DPPH22+) restores the triplet deactivation pathway, thereby activating its ability to generate reactive oxygen species (ROS). We have employed the ExBox4+-bound DPP complex (ExBox4+⊃DPP) for the safe delivery of DPP into the lysosomes of the cancer cells, imaging the cells by utilizing the fluorescence of the released DPPH22+, and regulating photodynamic therapy to kill cancer cells with high efficiency.
               
Click one of the above tabs to view related content.