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We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we… Click to show full abstract
We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.
Keywords:
quantification proteins;
proteins transcripts;
multiplexed quantification;
transcripts single;
single cells
Journal Title: Nature Biotechnology
Year Published: 2017
Link to full text (if available)
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Journal Title: Nature Biotechnology
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!