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Imaging: Volumetric functional imaging two ways

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stereoscopy (vTwINS), another method for fast volumetric functional brain imaging. The method is conceptually similar to Ji’s in that it uses an elongated point-spread function (PSF) to gain volume information.… Click to show full abstract

stereoscopy (vTwINS), another method for fast volumetric functional brain imaging. The method is conceptually similar to Ji’s in that it uses an elongated point-spread function (PSF) to gain volume information. In vTwINs, the elongated PSF is split, separated and angled inward to create a “V” shape. When scanned, this PSF yields a 2D projection image that can be used to extract depth information. An advantage of this approach is that it is useful for imaging densely labeled samples. The team demonstrated the power of their approach by carrying out large-scale recording of calcium transients in the visual cortex and hippocampus of awake mice. Rita Strack

Keywords: functional imaging; volumetric functional; imaging volumetric; two ways; imaging two

Journal Title: Nature Methods
Year Published: 2017

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