LAUSR

Since the outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), more than 6 million cases are confirmed and over 300,000 cases are dead after infection. Dysfunction of immunity in COVID-19 patients has been considered as one of the fatal factors for patients, especially cytokine release syndrome and lymphopenia. The reduced number and increased exhaustion level of lymphocyte are associated with elevated inflammatory cytokines in COVID-19 patients. However, the mechanism of cytokineinduced lymphopenia in COVID-19 is very unclear. IL-2 is critical for the proliferation, differentiation, and function of T cells, including Tregs, CD4, and CD8 effector cells. Here, we reported the negative relationship between the concentration of soluble IL2 receptor (sIL-2R) and T-cell number in blood from COVID-19 patients. In vitro addition of recombinant CD25 could inhibit the proliferation and function of T cells from PBMC after stimulated with TCR signaling, which could be rescued by strong IL-2 signaling. Our data suggested the importance of IL-2 signaling in lymphopenia of COVID-19 patients. In the previous report, we have reported that plasma cytokines including sIL-2R, IL-6, TNF-a, and IL-10 concentrations on admission were significantly higher in severe cases than moderate cases. To investigate the correlation between plasma cytokines and lymphocytes in blood from COVID-19 patients, the data that has a one -to-one relationship between the cytokines and lymphocytes from 11 samples of 9 patients were analyzed. Among those patients, two patients (patient 8 and patient 9) were from the patients included in our previous report. The remaining seven patients (from patient 1 to patient 7) with available dynamic data of cytokines and lymphocytes subset counts were consecutive patients admitted from February 18–22, 2020. Samples were collected as shown in Supplementary Fig. 1a. We divided the samples into two groups (one group contained samples collected within 10 days after illness onset and another group contained samples collected between 10 and 20 days after illness onset). We found that the longer it took after illness onset, the less percentage of CD3 and CD8 T cells was (Fig. 1a). HLADR molecules are important surface activation markers for T cells. We also found the impaired HLA-DR CD3 T cells in long-term onset group (Fig. 1a), whose CD4 T cells, CD19 B cells and NK cells percentage were not reduced when compared with shortterm onset group (Supplementary Fig. 1b). Through analyzing the time axis, we found that sIL-2R was positively related to days after illness onset, but not TNF-a, IL-10 and IL-6 (Fig. 1b and Supplementary Fig. 1c). The sIL-2R (including 3 subunits of IL-2R: CD25, CD122, and CD132) was measured using the sandwich enzyme-linked immune-sorbent assay (ELISA). The concentration of sIL-2R in plasma from COVID-19 patients was much higher than healthy controls (Fig. 1c). Circulating sIL-2R has been shown to regulate T-lymphocyte activation in various immunological disorders and increased sIL-2R concentration in plasma predicts a decreased cellular response to IL-2. Serum levels of sIL-2R are significantly higher in Kawasaki disease patients, who suffer a systemic inflammatory disease closely associated with infections. The latest clinical findings that a pediatric patient diagnosed and treated for Kawasaki disease in the setting of confirmed COVID-19 infection indicate the underlying connection between the two diseases and the potential function of sIL-2R in COVID-19. Combined with the important function of IL-2 signaling in T cells, we speculated the important function of sIL-2R during the onset of lymphopenia in COVID-19 patients. Induced Ki67 expression stimulated with anti-CD3/28 antibodies among CD3/CD4/CD8 T cells in PBMC from a healthy donor was decreased in the high concentration of recombinant CD25, which could be rescued by strong IL-2 signaling (Fig. 1d, SFig. 1d, e). However, recombinant CD25 mediated IL-2 signaling inhibition was not involved in cell death in T cells (Fig. 1e). Flow cytometric analysis showed that IFN-gamma, but not IL-2 expression in T cells was impaired with a high concentration of recombinant CD25 (Fig. 1f, g ), which was consistent with the previous report that soluble CD25 could inhibit the proliferation and function of T cells. Correlation analysis between sIL-2R concentration and percentage of different immune cell types in COVID-19 patients suggested that sIL-2R might be a negative regulatory factor for T cells, especially CD8+ T cells (Fig. 1h), but not CD4+ T cells, NK cells, and B cells (Supplementary Fig. 1f). The situation of COVID-19 patients is more complicated than we thought. We believe that a high concentration of sIL-2R makes a contribution to lymphopenia in COVID-19, but we do not know whether it is a decisive factor or not. Lymphopenia has been considered to