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A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

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Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine… Click to show full abstract

Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.A microfluidics-based assay for in situ chemotaxis experiments.

Keywords: situ chemotaxis; chemotaxis assay; microfluidics based; study behaviour; assay study; based situ

Journal Title: Nature Microbiology
Year Published: 2017

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