LAUSR

Astrocytes functionally interact with neurons and with other brain cells. Although not electrically excitable, astrocytes display a complex repertoire of intracellular Ca 2+ signalling that evolves in space and time within single astrocytes and across astrocytic networks. Decoding the physiological meaning of these dynamic changes in astrocytic Ca 2+ activity has remained a major challenge. This Review describes experimental preparations and methods for recording and studying Ca 2+ activity in astrocytes, focusing on the analysis of Ca 2+ signalling events in single astrocytes and in astrocytic networks. The limitations of existing experimental approaches and ongoing technical and conceptual challenges in the interpretation of astrocytic Ca 2+ events and their spatio-temporal patterns are also discussed. Although not electrically excitable, astrocytes display a complex repertoire of intracellular Ca 2+ signalling. Semyanov, Henneberger and Agarwal describe experimental preparations and methods for studying Ca 2+ activity in astrocytes, their limitations and the ongoing technical and conceptual challenges in the interpretation of astrocytic Ca 2+ events and their spatio-temporal patterns.