Photo by museumsvictoria from unsplash
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice… Click to show full abstract
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner.
Keywords:
high efficiency;
editing optimized;
optimized paired;
prime editing;
efficiency;
efficiency prime
Journal Title: Nature biotechnology
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!
Journal Title: Nature biotechnology
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!