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MHz data collection of a microcrystalline mixture of different jack bean proteins

We provide a detailed description of a serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is the first high repetition rate XFEL… Click to show full abstract

We provide a detailed description of a serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is the first high repetition rate XFEL delivering MHz X-ray pulse trains at 10 Hz. The short spacing (<1 µs) between pulses requires fast flowing microjets for sample injection and high frame rate detectors. A data set was recorded of a microcrystalline mixture of at least three different jack bean proteins (urease, concanavalin A, concanavalin B). A one megapixel Adaptive Gain Integrating Pixel Detector (AGIPD) was used which has not only a high frame rate but also a large dynamic range. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development and for data analysis training for prospective XFEL users.Design Type(s)protocol testing objectiveMeasurement Type(s)protein structure dataTechnology Type(s)x ray crystallographyFactor Type(s)Sample Characteristic(s)CanavaliaMachine-accessible metadata file describing the reported data (ISA-Tab format)

Keywords: different jack; jack bean; microcrystalline mixture; bean proteins; ray

Journal Title: Scientific Data
Year Published: 2019

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