LAUSR

Polyadenylation plays an important role in gene regulation, thus affecting a wide variety of biological processes. In the rice blast fungus Magnaporthe oryzae the cleavage factor I protein Rpb35 is required for pre-mRNA polyadenylation and fungal virulence. Here we present the bioinformatic approach and output data related to a global survey of polyadenylation site usage in M. oryzae wild-type and Δ rbp35 strains under a variety of nutrient conditions, some of which simulate the conditions experienced by the fungus during part of its infection cycle. Design Type(s) transcription profiling by high throughput sequencing design • stimulus or stress design • replicate design • genetic modification design Measurement Type(s) total RNA Technology Type(s) polyA-selected RNA sequencing assay Factor Type(s) Organism Strain • culture medium • biological replicate Sample Characteristic(s) Magnaporthe oryzae Machine-accessible metadata file describing the reported data (ISA-Tab format)