Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization… Click to show full abstract
Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3'-terminal 2'-O-methylated miRNAs.
               
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