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Whole-cell catalysts overexpressing two enzymes for a double reduction cascade in which aliphatic α-branched α,β-unsaturated aldehydes are converted into Guerbet alcohols as a highly demanded class of lubricants were constructed… Click to show full abstract
Whole-cell catalysts overexpressing two enzymes for a double reduction cascade in which aliphatic α-branched α,β-unsaturated aldehydes are converted into Guerbet alcohols as a highly demanded class of lubricants were constructed and characterized. The reaction conditions were optimized to suppress by-product formation, achieving high conversion and selectivity. Application of methanol as a co-solvent on a 500 mL scale enabled a substrate concentration of 18.5 g L−1 obtaining 97% yield and 88% purity without further distillation. Addressing the needs of industrial processes, substrate loading was increased up to 94 g L−1 and the E-factor (environmental factor) was significantly improved compared to the original process with isolated enzymes.
Journal Title: Green Chemistry
Year Published: 2017
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