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Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system.

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We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3' cap-independent translation element (3' CITE) with no major hybridization switches… Click to show full abstract

We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3' cap-independent translation element (3' CITE) with no major hybridization switches in a plant expression system (wheat germ extract).

Keywords: cite; expression system; wheat germ; translation

Journal Title: Molecular bioSystems
Year Published: 2017

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