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Templated chemical ligation of 5'-amino and 3'-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase… Click to show full abstract
Templated chemical ligation of 5'-amino and 3'-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase and transcribed to make RNA. We also show that phosphoramidate ligation and orthogonal CuAAC-mediated DNA ligation can be used simultaneously.
Keywords:
tube gene;
chemical ligation;
ligation;
single tube
Journal Title: Chemical communications
Year Published: 2017
Link to full text (if available)
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Journal Title: Chemical communications
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!