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Visualization of vesicular transport from the endoplasmic reticulum to lysosome using an amidine derived two-photon probe.

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We report an amidine-based small molecule two-photon fluorescent probe (ELP1) for monitoring vesicle transport from the ER to lysosome in live cells. Two-photon microscopy imaging studies indicated that this probe… Click to show full abstract

We report an amidine-based small molecule two-photon fluorescent probe (ELP1) for monitoring vesicle transport from the ER to lysosome in live cells. Two-photon microscopy imaging studies indicated that this probe initially localized in the ER and subsequently transferred to lysosomal compartments through vesicular transport. These results may provide an effective tool for studying trafficking-related biology and pathology in real-time.

Keywords: photon; vesicular transport; two photon; probe; visualization vesicular

Journal Title: Chemical communications
Year Published: 2017

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