We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging… Click to show full abstract
We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.
Keywords:
sypher3s genetically;
enhanced brightness;
probe;
genetically encoded;
encoded fluorescent;
fluorescent ratiometric
Journal Title: Chemical communications
Year Published: 2018
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Journal Title: Chemical communications
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!