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Improved performance of immobilized lipase by interfacial activation on Fe3O4@PVBC nanoparticles

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An effective strategy for enhancement of catalytic activity and stability of immobilized lipase by interfacial activation on Fe3O4@PVBC (Fe3O4@polyvinylbenzyl chloride) nanoparticles was developed, which involved the fabrication of core–shelled Fe3O4@PVBC… Click to show full abstract

An effective strategy for enhancement of catalytic activity and stability of immobilized lipase by interfacial activation on Fe3O4@PVBC (Fe3O4@polyvinylbenzyl chloride) nanoparticles was developed, which involved the fabrication of core–shelled Fe3O4@PVBC nanoparticles via an emulsion polymerization process and the subsequent immobilization of lipase in phosphate buffer. Due to the magnetic nature of Fe3O4 cores and the presence of chloromethyl-functionalized polymer shells, the Fe3O4@PVBC nanoparticles were employed as valid magnetic carriers for lipase immobilization. Bradford assays indicated that the loading amount of lipase on the Fe3O4@PVBC nanoparticles was calculated to be 162.5 mg protein per g particles. The catalytic activity of the immobilized lipase retained about 99.6 ± 3.3% of the free enzyme activity, which was attributed to interfacial activation of lipase by Fe3O4@PVBC nanoparticles. Thermal and urea tolerance tests revealed that the immobilized lipase exhibited much better stabilities. Additionally, the immobilized lipase retained more than 69.8% of its initial activities after 10 times of reuse. It is believed that the results of the present investigation may provide a versatile approach for designing and fabricating biocatalysts with high activation and stability.

Keywords: immobilized lipase; pvbc nanoparticles; lipase; fe3o4 pvbc

Journal Title: RSC Advances
Year Published: 2017

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