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A novel three-fluorophore system as a ratiometric sensor for multiple protease detection.

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A synthetic three-fluorophore system with two enzymatically cleavable linkers has been developed for the simultaneous detection of two proteases in a mixture. The probe was designed to afford single excitation/triple… Click to show full abstract

A synthetic three-fluorophore system with two enzymatically cleavable linkers has been developed for the simultaneous detection of two proteases in a mixture. The probe was designed to afford single excitation/triple emission ratiometric detection through a fluorescence change during the cleavage of a peptide linker. The developed assays were verified for trypsin and chymotrypsin as the model enzymes.

Keywords: detection; novel three; three fluorophore; fluorophore system

Journal Title: Chemical communications
Year Published: 2018

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