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Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs.

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Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag… Click to show full abstract

Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag peptides and small molecules were successfully utilized to detect antigens in a rapid and quantitative manner.

Keywords: antibody probes; fluorescent; selective terminal; fluorescent antibody; antigen responsive

Journal Title: Chemical communications
Year Published: 2018

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