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While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the… Click to show full abstract
While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold at a 0.1 target/probe ratio.
Keywords:
performance blocking;
improving nanocluster;
blocking unlabeled;
beacon;
nanocluster beacon;
beacon performance
Journal Title: Chemical communications
Year Published: 2019
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Journal Title: Chemical communications
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!