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Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage… Click to show full abstract
Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.
Keywords:
dnazyme powered;
acid release;
powered nucleic;
dnazyme;
release solid;
nucleic acid
Journal Title: Chemical communications
Year Published: 2019
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Journal Title: Chemical communications
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!