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The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols.

We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme… Click to show full abstract

We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.

Keywords: methionase chain; chain reaction; system detection; amplification

Journal Title: Chemical communications
Year Published: 2020

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