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Genetically fused charged peptides induce rapid crystallization of proteins.

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We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few… Click to show full abstract

We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

Keywords: fused charged; rapid crystallization; genetically fused; charged peptides; induce rapid

Journal Title: Chemical communications
Year Published: 2020

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