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Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements… Click to show full abstract
Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.
Keywords:
stoichiometric quantification;
assemblies qpaint;
spatially dense;
quantification spatially;
dense assemblies
Journal Title: Nanoscale
Year Published: 2019
Link to full text (if available)
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Journal Title: Nanoscale
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!