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We describe a versatile CRISPR/Cas-based strategy to construct multi-enzyme complexes scaffolded on a DNA template in programmable patterns. Catalytically inactive dCas9 nuclease was used in combination with SpyCatcher-SpyTag chemistry to… Click to show full abstract
We describe a versatile CRISPR/Cas-based strategy to construct multi-enzyme complexes scaffolded on a DNA template in programmable patterns. Catalytically inactive dCas9 nuclease was used in combination with SpyCatcher-SpyTag chemistry to assemble enzymes in a highly modular fashion. Five enzymes comprising the violacein biosynthesis pathway were precisely organized in nanometer proximity; a notable increase in violacein production demonstrated the benefits of scaffolding.
Keywords:
multi enzyme;
crispr cas;
enzyme complexes;
cas directed
Journal Title: Chemical communications
Year Published: 2020
Link to full text (if available)
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Journal Title: Chemical communications
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!