Photo from wikipedia
We develop a label-free fluorescence method for ultrasensitive detection of apoptosis-associated caspase activity based on branched rolling circle amplification. This work demonstrates for the first time the use of a… Click to show full abstract
We develop a label-free fluorescence method for ultrasensitive detection of apoptosis-associated caspase activity based on branched rolling circle amplification. This work demonstrates for the first time the use of a nucleic acid amplification strategy for the amplified detection of caspase activity, which allows for highly sensitive detection of endogenous caspase even at the single-cell level. This method can be further applied for kinetic analysis and inhibitor screening.
Keywords:
caspase;
detection;
caspase activity;
label free;
amplification
Journal Title: Chemical communications
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!
Journal Title: Chemical communications
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!