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A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection.

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An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels… Click to show full abstract

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively.

Keywords: intracellular signal; atp single; quantification atp; amplification strategy; single cells; signal amplification

Journal Title: Chemical communications
Year Published: 2020

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