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A recombinant HALO-GFP fusion protein was designed and isolated to demonstrate the feasibility of controlling the number and orientation of protein ligands to be conjugated on colloidal gold nanoparticles. AuNPs… Click to show full abstract
A recombinant HALO-GFP fusion protein was designed and isolated to demonstrate the feasibility of controlling the number and orientation of protein ligands to be conjugated on colloidal gold nanoparticles. AuNPs functionalized with exactly one or exactly two GFP molecules exhibited fully preserved functionality of the protein. The method is very straightforward and generally provides highly bioactive nanoparticle-protein conjugates.
Keywords:
discrete number;
number ligands;
nanoparticles discrete;
number;
colloidal nanoparticles;
functionalization colloidal
Journal Title: Chemical communications
Year Published: 2020
Link to full text (if available)
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Journal Title: Chemical communications
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!