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Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe.

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Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up… Click to show full abstract

Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.

Keywords: assessing changes; expression; changes expression; expression levels; cell surface; levels cell

Journal Title: Chemical communications
Year Published: 2021

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