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Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes.

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We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His6-tagged eGFP and four His6-tagged… Click to show full abstract

We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His6-tagged eGFP and four His6-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His6-tagged HRP.

Keywords: complex mediated; iii complex; cell surface; cell; his6 tagged

Journal Title: Chemical communications
Year Published: 2021

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