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A sequentially activated bioluminescent probe for observation of cellular H2O2 production induced by cysteine.

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We report herein a caged luciferin probe Cy-Hy as a sequentially activated probe to selectively and sensitively sense L-Cys and H2O2. The probe displayed fluorescence and bioluminescence responses toward the… Click to show full abstract

We report herein a caged luciferin probe Cy-Hy as a sequentially activated probe to selectively and sensitively sense L-Cys and H2O2. The probe displayed fluorescence and bioluminescence responses toward the two analytes. Utilizing the present probe, cellular excess L-Cys-induced H2O2 up-regulation was observed for the first time in living MDA-MB-231 cells.

Keywords: probe observation; activated bioluminescent; probe; sequentially activated; h2o2; bioluminescent probe

Journal Title: Chemical communications
Year Published: 2021

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