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We demonstrate for the first time the simultaneous measurement of the acetyltransferase (HAT) and crotonyltransferase (HCT) activities of histone acetylation writer p300 by integrating antibody-based fluorescence labeling with single molecule… Click to show full abstract
We demonstrate for the first time the simultaneous measurement of the acetyltransferase (HAT) and crotonyltransferase (HCT) activities of histone acetylation writer p300 by integrating antibody-based fluorescence labeling with single molecule detection. This methods exhibits good specificity and high sensitivity. Moreover, it can accurately evaluate the kinetic parameters of both the HAT and HCT activities of p300 and screen inhibitors.
Keywords:
histone acetylation;
single molecule;
writer p300;
activities histone;
molecule detection;
acetylation writer
Journal Title: Chemical communications
Year Published: 2021
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Journal Title: Chemical communications
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!