LAUSR

The demand for proteins with high purity, high activity and high stability is increasing, especially in the biopharmaceutical industry. Traditional industrial protein production technologies include chromatography, which is expensive, and salting out and the traditional crystallization method, which require large amounts of salt and have an adverse effect on the environment. In recent years, a new method—solvent freeze-out (SFO) technology for protein crystallization—has been developed. Compared with the traditional methods, SFO requires less salt, and can be carried out at low temperatures, resulting in a more stable and active protein. In addition, SFO can control the nucleation and crystal growth process under different supersaruation levles by setting appropriate operating temperature, which can ensure industrial application of this technology. This paper reviews important advances in SFO technology for the crystallization of proteins.