LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Chiral carbon dots - a functional domain for tyrosinase Cu active site modulation via remote target interaction.

Photo from wikipedia

The nano-hybrid enzyme is an ideal catalytic system that integrates various advantages from biocatalysis and nanocatalysis into homogeneous and heterogeneous catalysis. However, great efforts are still needed to fully understand… Click to show full abstract

The nano-hybrid enzyme is an ideal catalytic system that integrates various advantages from biocatalysis and nanocatalysis into homogeneous and heterogeneous catalysis. However, great efforts are still needed to fully understand the interactions between nanoparticles and enzymes. Here, we show chiral carbon dots (CDs) as a new functional domain for tyrosinase Cu active site modulation via remote target interaction. Three kinds of chiral CDs (LCDs-1/-2/-3; DCDs-1/-2/-3) were fabricated by thermal treatment of citric acid and L/D-aspartic acid. Then a series of CDs/tyrosinase composites (namely, nano-hybrid-enzymes) were prepared, demonstrating good regulation of enzyme catalytic kinetics. Especially, we find that LCDs-1 is an irreversible inhibitor with great inhibition effect while the others are all reversible inhibitors. Furthermore, it is suggested by both experiments and all-atom molecular dynamics simulations that the joint effect of LCDs-1 and tyrosinase makes LCDs-1 serve as a new functional domain, which has a distinguished ability to control the conformational changes of the key sites of the active center of the tyrosinase (e.g., H60) and thus the escaping behavior of copper ions and the catalytic activity. This work opens a new route for nano-hybrid-enzyme design and enzyme activity regulation with chiral carbon materials as functional domains via remote target interaction.

Keywords: via remote; chiral carbon; remote target; functional domain; tyrosinase; target interaction

Journal Title: Nanoscale
Year Published: 2022

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.