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DNA nanomachine for visual detection of structured RNA and double stranded DNA.

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Visual detection of ssRNA and dsDNA amplicons was achieved at room temperature without the need for a probe-analyte annealing stage. This approach uses a DNA nanostructure equipped with two analyte-binding… Click to show full abstract

Visual detection of ssRNA and dsDNA amplicons was achieved at room temperature without the need for a probe-analyte annealing stage. This approach uses a DNA nanostructure equipped with two analyte-binding arms. Highly selective binding of the third arm leads to the formation of a G-quadruplex structure capable of changing the solution color.

Keywords: dna; nanomachine visual; visual detection; detection structured; dna nanomachine

Journal Title: Chemical communications
Year Published: 2022

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