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We introduce a novel electrochemical protein quantitation based on the shotgun biotin tagging of proteins prior to their interfacial immunocapture and polymeric enzyme tagging. The highly amplified faradaic signals generated… Click to show full abstract
We introduce a novel electrochemical protein quantitation based on the shotgun biotin tagging of proteins prior to their interfacial immunocapture and polymeric enzyme tagging. The highly amplified faradaic signals generated from a novel ferrocene-tyramine adduct enable fg mL-1 (attomolar) levels of detection and span cross a 5 orders of magnitude dynamic range. This work supports ultrasensitive protein marker detection in a single antibody immunoassay format.
Keywords:
shotgun;
faradaic shotgun;
faradaic;
shotgun tagging;
catalysed amplification;
amplification faradaic
Journal Title: Chemical communications
Year Published: 2022
Link to full text (if available)
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Journal Title: Chemical communications
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!