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NAPT, an unbiased approach for sequential analysis of the protein N- and C-terminome.

Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the… Click to show full abstract

Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the Protein N- and C-Terminome (NAPT) for selective separation of protein termini. Altogether, we identified 2458 protein N-termini and 3056 protein C-termini from the HeLa cell line.

Keywords: terminome napt; protein terminome; analysis protein; protein termini; protein

Journal Title: Chemical communications
Year Published: 2022

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