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A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation… Click to show full abstract
A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation of a cleavable linker via the click reaction, achieving 8-oxoGua pull-down and sequencing. Using APSC-8-oxoGua-seq, 8-oxoGua can be identified at single-base resolution.
Keywords:
oxo dihydroguanine;
microrna transcriptome;
chemical labeling;
achieves oxo;
chemical;
labeling achieves
Journal Title: Chemical communications
Year Published: 2023
Link to full text (if available)
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Journal Title: Chemical communications
Year Published: 2023
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!