LAUSR

We consider experimentally the Takatori-Sahu model of vesicle shape fluctuations induced by enclosed active matter, a model till present tested only in the absence of collective motion because few enclosed bacteria were used to generate the desired active motion (S. C. Takatori and A. Sahu, Phys. Rev. Lett., 2020, 124, 158102). Using deformable giant unilamellar vesicles (GUVs) and phase contrast microscopy, we extract the mode-dependence of GUV shape fluctuations when hundreds of E. coli bacteria are contained within each GUV. In the microscope focal plane, patterns of collective bacteria flow include vortex flow, dipolar flow, and chaotic motion, all of which influence the GUV shapes. The Takatori-Sahu model generalizes well to this situation if one considers the moving element to be the experimentally-determined size of the collecively-moving flock.