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A disordered tether to iLID improves photoswitchable protein patterning on model membranes.

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Reversible protein patterning on model membranes is important to reproduce spatiotemporal protein dynamics in vitro. An engineered version of iLID, disiLID, with a disordered domain as a membrane tether improves… Click to show full abstract

Reversible protein patterning on model membranes is important to reproduce spatiotemporal protein dynamics in vitro. An engineered version of iLID, disiLID, with a disordered domain as a membrane tether improves the recruitment of Nano under blue light and the reversibility in the dark, which enables protein patterning on membranes with higher spatiotemporal precision.

Keywords: protein patterning; model membranes; protein; patterning model; disordered tether

Journal Title: Chemical communications
Year Published: 2023

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