LAUSR

BACKGROUND Morbidity of chronic kidney disease (CKD) is increased, with many complications and high mortality rates. The characteristics of oral microbiome in CKD patients have not been reported. This study aims to analyze the oral microbiome, and to demonstrate the potential of microbiome as non-invasive biomarkers for CKD patients. METHODS The study collected 253 oral samples from different regions of China (Central China and East China) prospectively and finally 235 samples completed Miseq sequencing, including 103 samples from CKD patients and 132 healthy controls (HCs). RESULTS Compared with HCs (n=88), the oral microbial diversity in CKD patients (n=44) was increased. Foureen genera including Streptococcus, Actinomyces and Leptotrichia wereenriched, while 6 genera including Prevotella, Haemophilus were decreased in CKD patients. Moreover, 49 predicted microbial gene functions including arginine metabolism and tryptophan metabolism increased, while 55 functions including Ribosome and DNA repair recombination proteins decreased. Furthermore, correlation analysis demonstrated that 38 operational taxonomic units (OTUs) were closely related to 5 clinical indicators of CKD. Notably, 7 optimal biomarkers were identified using random forest model, and the classifier model respectively reached an area under the curve (AUC) of 0.9917 and 0.8026 in the discovery and validation phase, achieving a cross-region validation. CONCLUSIONS We first illustrated the characteristics of the oral microbiome of patients with CKD, identified the potential of oral microbial makers as non-invasive tools for the diagnosis of CKD, and achieved cross-region validation.