In the original paper,1 a calibration error exists in the image-formation model used to analyze experimental images taken by our microscope, causing a bias in the orientation measurements in Figs.… Click to show full abstract
In the original paper,1 a calibration error exists in the image-formation model used to analyze experimental images taken by our microscope, causing a bias in the orientation measurements in Figs. 2 and 3. The updated measurements are shown in Fig. E1; Figs. E1(a) and E1(b) correspond to the original Figs. 2(c) and 2(d); Figs. E1[c(i)] and E1[d(i)] correspond to the estimates in the original Figs. 3[a(iii)] and 3[b(iii)]; Figs. E1[c(ii)] and E1[d(ii)] correspond to the original Figs. 3[a(iv)] and 3[b(iv)]. We have also updated the supplementary material to discuss the revised PSF model and estimation algorithms (supplementary material 2) and show the revised model and measurements (Figs. S1, S3, S7, S8, and S10–S13).
               
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