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Parentage assignment using microsatellite DNA typing for the endangered numbat (Myrmecobius fasciatus)

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The numbat (Myrmecobius fasciatus) is an endangered and peculiar marsupial with a diet that consists almost exclusively of termites. This study developed a parentage-testing system for numbats using microsatellite markers.… Click to show full abstract

The numbat (Myrmecobius fasciatus) is an endangered and peculiar marsupial with a diet that consists almost exclusively of termites. This study developed a parentage-testing system for numbats using microsatellite markers. Nineteen loci detected 143 alleles, with 4–13 alleles/locus and average expected heterozygosity of 77% (range 0.665–0.855). The total parentage exclusion probability was >0.9999 (given only the genotype of the offspring), >0.9999 for excluding a candidate parent from the parentage of an arbitrary offspring (given the genotype of the offspring and parent) and the probability of identity for full-sibs was 4.6 × 10–9. Overall, these microsatellites offer a simple and highly informative marker-set for parentage identification in numbats.

Keywords: using microsatellite; parentage; numbat myrmecobius; myrmecobius fasciatus

Journal Title: Australian Mammalogy
Year Published: 2020

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