LAUSR

Significance Organisms often generate benefits shared among their whole group, but such cooperation is vulnerable to collapse if individuals can instead benefit by exploiting the cooperation of others. While relatedness can promote cooperation, many species lack reliable mechanisms to ensure high relatedness. They are therefore vulnerable to a breakdown of cooperation unless they are able to enforce cooperation. We test this idea through experimental manipulation of group composition in a social microbe. We find that groups avert the expected collapse in cooperation at low relatedness due to inadvertent enforcement of cooperation by a mechanism that prevents errors in multicellular development. Our findings explain how mechanisms that promote cooperation can arise as by-products of natural selection acting on traits in other contexts.