LAUSR

Significance Urbanization is rapidly transforming Southeast Asia, altering the landscape and the interactions between people, animals, and the environment. These changes have the potential to exacerbate many existing health challenges in the region, including those posed by zoonoses. Here, we used a novel, multidisciplinary, ecosystem-level approach to examine the influence of urbanization on zoonotic disease risk in a Southeast Asian city. We infer that urbanization alters the ecology of animal reservoirs, arthropod vectors, and pathogens in a manner that may increase transmission risk from multiple zoonotic diseases in urban areas. This effect was particularly strong for pathogens associated with environmental or tick-borne transmission, providing targets for the development of low-cost interventions to reduce zoonotic disease risk in tropical cities.