LAUSR

Significance Phase transitions in materials are accompanied by drastic changes in their properties. Systems abruptly become softer, more conductive, have better heat storage, or support chemical reactions more efficiently. Since changes take place over small variations in external conditions (tension, temperature, pH, calcium), they appear like an on/off switch. Here, we provide experimental evidence that membrane patches of single living cells can go through a reversible phase transition. It is extremely “sharp” (highly nonlinear), and from a thermodynamic point of view we conclude it cannot only be triggered by temperature but also by pH changes (as produced by enzymes). The results strongly support the idea that phase transitions may be a tool for living systems to control their functions even specifically. The origin of nonlinear responses in cells has been suggested to be crucial for various cell functions including the propagation of the nervous impulse. In physics, nonlinear behavior often originates from phase transitions. Evidence for such transitions on the single-cell level, however, has so far not been provided, leaving the field unattended by the biological community. Here, we demonstrate that single cells of a human neuronal cell line display all optical features of a sharp, highly nonlinear phase transition within their membrane. The transition is reversible and does not originate from protein denaturation. Triggered by temperature and modified by pH here, a thermodynamic approach strongly suggests that similar nonlinear state changes can be induced by other variables such as calcium or mechanical stress. At least in lipid membranes, such state changes are accompanied by significant changes in permeability, enzyme activity, elastic, and electrical properties.