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Significance Hoogsteen base pairs, which are thermodynamically less stable than the canonical Watson-Crick base pairs, often feature in complexes of DNA with proteins and small molecules, where they play key… Click to show full abstract
Significance Hoogsteen base pairs, which are thermodynamically less stable than the canonical Watson-Crick base pairs, often feature in complexes of DNA with proteins and small molecules, where they play key roles in DNA recognition, repair, and replication. Distinguishing between Hoogsteen and Watson-Crick base pairs in large DNA complexes and assemblies presents significant challenges for traditional structural biology techniques. Here, we demonstrate that Hoogsteen base pairs can be detected if present and distinguished from Watson-Crick base pairs in a wide range of DNA systems based on characteristic NMR chemical shifts and dipolar couplings by using multidimensional dynamic nuclear polarization-enhanced solid-state NMR spectroscopy.
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Year Published: 2022
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