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Significance Measuring active cells that contribute to soil antimicrobial resistance (AMR) is critical for understanding AMR behavior in ecosystems. Here, single-cell Raman with isotope labeling was developed to profile active… Click to show full abstract
Significance Measuring active cells that contribute to soil antimicrobial resistance (AMR) is critical for understanding AMR behavior in ecosystems. Here, single-cell Raman with isotope labeling was developed to profile active antibiotic-resistant bacteria (ARB) inhabiting different soils. Their abundance and activity in soils increased with anthropogenic activities. Further targeted single-cell sorting and metagenomics pinpoint “who is doing what and how” in the most active ARB. Several uncultured genera and a pathogenic strain were identified, along with the underlying resistance genes and mobile genetic elements. This approach linking the resistant phenome to the genome is highly valuable for assessing AMR risk and in guiding human activities against AMR.
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Year Published: 2022
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