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Significance Cells respond to shifts in glucose supply or fluctuations in energetic demand through regulated changes in glycolytic metabolism. The rate at which glucose is converted to pyruvate is of… Click to show full abstract
Significance Cells respond to shifts in glucose supply or fluctuations in energetic demand through regulated changes in glycolytic metabolism. The rate at which glucose is converted to pyruvate is of considerable importance to cellular physiology; however, measuring this property at single-cell resolution is fundamentally difficult. The glycolytic intermediate fructose 1,6-bisphosphate (FBP) exhibits large changes in concentration that track with changes in flux. We developed a fluorescent biosensor for FBP exploiting this correlation to monitor rapid changes in glycolysis within single cells. HYlight was used in pancreatic β-cells to uncover aspects of glycolytic regulation, dynamics and heterogeneity. Given the conservation and importance of glycolysis, we believe this new biosensor will be broadly useful in a variety of tissues and organisms.
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Year Published: 2022
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